GPC1 抗体 (Internal Region)
Quick Overview for GPC1 抗体 (Internal Region) (ABIN349638)
抗原
See all GPC1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 200-401-b44
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Supplier
- Rockland
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原理
- Glypican 1 Antibody
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交叉反应 （详细）
- This antibody is specific for human glypican-1 protein.
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产品特性
- Synonyms: rabbit anti-Glypican-1 Antibody, Glypican 1, Glypican1, Secreted glypican-1, GPC1, FLJ38078
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纯化方法
- Anti-Glypican-1 was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Glypican-1 protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Glypican 1 (GPC1) antibody
KO Validated GPC1 适用: 人 WB, ELISA, ICC, IP, IF, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 155-292) antibodyGPC1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 342-530) antibodyGPC1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 24-131) antibodyGPC1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 12-41), (N-Term) antibodyGPC1 适用: 人 WB, IHC (p), FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 301-400) antibodyGPC1 适用: 人 ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 114-277) antibodyGPC1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 343-545) antibodyGPC1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (Internal Region) antibodyVerified GPC1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 290-420) antibodyGPC1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 10 μg/mL
Application Note: Anti-Glypican-1 antibody has been tested for use in ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~61 kDa in size corresponding to glypican by western blotting in the appropriate cell lysate or extract. The higher molecular weight (110 kDa) of transfected Fc-glypican compared with the expected MW of glypican is likely due to the presence of the Fc-tag.
Western Blot Dilution: 1:500- 1:2,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- GPC1 (Glypican 1 (GPC1))
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别名
- GPC1
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背景
- Background: Glypican-1 antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Glypican-1 (also known as GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS). This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI). Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth. In humans, glypican-1 is over expressed in breast and brain cancers (gliomas). All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor. The amino acid sequences of the six vertebrate glypican family members vary from 17 % to 63 % identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican-1 has been shown to interact with SLIT2.
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基因ID
- 2817, 30410985
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UniProt
- P35052
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途径
- Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation
抗原
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