CENPQ 抗体
Quick Overview for CENPQ 抗体 (ABIN349636)
抗原
See all CENPQ 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-401-b48
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Supplier
- Rockland
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原理
- CENP-Q Antibody
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交叉反应 （详细）
- This antibody is specific for human CENP-Q protein.
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产品特性
- Synonyms: rabbit anti-CENP-Q Antibody, CenpQ, centromere protein Q, CENP Q
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纯化方法
- This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length human CENP-Q recombinant protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Centromere Protein Q (CENPQ) (AA 1-268) antibody
CENPQ 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2B5-2D12 unconjugated
anti-Centromere Protein Q (CENPQ) (N-Term) antibodyCENPQ 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein Q (CENPQ) (AA 131-268) antibodyCENPQ 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein Q (CENPQ) antibodyCENPQ 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein Q (CENPQ) (AA 35-84) antibodyCENPQ 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein Q (CENPQ) (N-Term) antibodyCENPQ 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This protein A purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26-31 kDa in size corresponding to human CENP-Q by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:100 - 1:500
ELISA Dilution: 1:5,000 1:20,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.15 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CENPQ (Centromere Protein Q (CENPQ))
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别名
- CENPQ
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cenp-Q (also known as centromere protein Q or CENPQ) is a nuclear/centromeric protein that is one of the critical components that constitutes the CENP-O complex at the kinetochores and appears to stabilize PBIP1/CENP-U(50)/MLF1IP in the complex. This complex is important for proper recruitment of polo-like kinase 1 (Plk1) to the mitotic kinetochores. A failure in this process results in improper microtubule attachment to the kinetochores and chromosome missegregation that ultimately lead to aneuploidy.
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基因ID
- 55166, 40068061
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UniProt
- Q7L2Z9
抗原
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