GM-CSF 抗体
Quick Overview for GM-CSF 抗体 (ABIN349632)
抗原
See all GM-CSF (CSF2) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 210-401-314
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Supplier
- Rockland
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原理
- GM-CSF Antibody
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交叉反应 （详细）
- In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse GM-CSF in cell supernatants and certain body fluids.
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产品特性
- Synonyms: rabbit anti-GM-CSF antibody, rabbit anti-Granulocyte-Macrophage colony-stimulating factor antibody, CSF, sargramostim, molgramostin
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纯化方法
- This purified antibody has been purified by a multi-step process which includes delipidation, salt fractionation, and heat treatment to 56°C for 30 minutes.
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免疫原
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Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse GM-CSF protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 71-141) antibody
CSF2 适用: Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 65-144) antibodyCSF2 适用: 人 WB, ELISA, IF, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) antibodyCSF2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 59-85) antibodyCSF2 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB23360 unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) antibodyCSF2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 19-141) antibodyCSF2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibodyCSF2 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) antibodyCSF2 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 62-89), (Middle Region) antibodyCSF2 适用: 人 WB, FACS, IF, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) antibodyCSF2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This purified antibody has been tested in western blot and is suitable for use in neutralizations and ELISA. Reactivity is also expected in radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 14.3 kDa in size corresponding to mouse GM-CSF protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Neutralization Dilution: 1:400
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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别名
- Csf2
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背景
- Background: Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF, Colony-stimulating factor, CSF, sargramostim and molgramostin) is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors show significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.
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基因ID
- 12981
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NCBI登录号
- NP_034099
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UniProt
- P01587
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
抗原
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