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DYKDDDDK Tag 抗体 (HRP)

Name: DYKDDDDK Tag 抗体 (HRP)
SKU: ABIN349598
Availability: OutOfStock

This anti-DYKDDDDK Tag antibody is a 兔多克隆 antibody detecting DYKDDDDK Tag in WB, ELISA 和 IP. Suitable for . This Primary Antibody has been cited in 2+ publications.

Western blot of Peroxidase conjugated Rabbit Anti-DYKDDDDK same epitope as Sigma's Anti-FLAG antibody. (DYKDDDDK Tag 抗体 (HRP))

Western Blot showing detection of alpha tubulin from HeLa and NIH/3T3. (DYKDDDDK Tag 抗体 (HRP))

2 images

(2 references)

产品编号 ABIN349598

Supplier Product No.: 600-403-383

发货至: 中国

Datasheet as PDF

Quick Overview for DYKDDDDK Tag 抗体 (HRP) (ABIN349598)

抗原

DYKDDDDK Tag

适用

请咨询

宿主

兔

克隆类型

多克隆

标记

This DYKDDDDK Tag antibody is conjugated to HRP

应用范围

Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.

600-403-383

Supplier

Rockland

序列

DYKDDDDK

产品特性

Concentration Definition: by UV absorbance at 280 nm

免疫原

This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the carboxy terminal end to facilitate coupling. This antibody reacts with DYKDDDDK conjugated proteins.

亚型

IgG
anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF 宿主: 小鼠 unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated

anti-DYKDDDDK Tag antibody
适用: 标记 WB, ELISA, IP, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F11G1 unconjugated

anti-DYKDDDDK Tag antibody
适用: 人 WB 宿主: 兔 Polyclonal RB0493 unconjugated

anti-DYKDDDDK Tag antibody (HRP)
WB, ELISA, IF 宿主: 小鼠 Monoclonal 5A8E5 HRP

anti-DYKDDDDK Tag antibody
WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF, IC 宿主: 小鼠 Monoclonal unconjugated

应用备注

This antibody is optimally suited for monitoring the expression of DYKDDDDK tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the DYKDDDDK epitope. The antibody recognizes the epitope tag fused to the amino- and carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and DYKDDDDK containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK. polyclonal antibody to detect DYKDDDDK conjugated proteins binds DYKDDDDK containing fusion proteins with greater affinity than the widely used monoclonal M1, M2 and M5 clones, and shows greater sensitivity in most assays. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.

限制

仅限研究用
状态

Lyophilized

溶解方式

Restore with deionized water (or equivalent)

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Gentamicin sulfate

注意事项

Do NOT add Sodium Azide!

储存条件

4 °C
Benjamin, van der Meer, Im, Plosa, Zaynagetdinov, Burman, Havrilla, Gleaves, Polosukhin, Deutsch, Yanagisawa, Davidson, Prince, Young, Blackwell: "Epithelial-Derived Inflammation Disrupts Elastin Assembly and Alters Saccular Stage Lung Development." in: The American journal of pathology, Vol. 186, Issue 7, pp. 1786-1800, (2017) (PubMed).

Saxon, Cheng, Han, Polosukhin, McLoed, Richmond, Gleaves, Tanjore, Sherrill, Barham, Yull, Blackwell: "p52 Overexpression Increases Epithelial Apoptosis, Enhances Lung Injury, and Reduces Survival after Lipopolysaccharide Treatment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 196, Issue 4, pp. 1891-9, (2016) (PubMed).
抗原

DYKDDDDK Tag

别名

Flag Tag

物质类

Tag

背景

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, DYKDDDDK and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
Synonyms: HRP