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Myc Tag 抗体 (HRP)

WB, IP, ELISA 宿主: 兔 Polyclonal HRP
产品编号 ABIN349597
发货至: 中国
  • 抗原 See all Myc Tag products
    Myc Tag
    适用
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    克隆类型
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    标记
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    This Myc Tag antibody is conjugated to HRP
    应用范围
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    Western Blotting (WB), Immunoprecipitation (IP), ELISA
    序列
    EQKLISEEDL
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    免疫原
    This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
    亚型
    IgG
  • 应用备注
    Anti-myc has utility to detect the fusion protein of the myc epitope cloned along with the target gene.   As such, anti-myc/myc can be used to identify fusion proteins containing the myc epitope.  The antibody recognizes the Myc tag fused either to the amino- or carboxy- termini of targeted proteins.  This antibody has been tested by ELISA and western blotting against both the immunizing peptide and myc containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Restore with deionized water (or equivalent)
    浓度
    1.0 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    储存液
    Gentamicin sulfate
    注意事项
    Do NOT add Sodium Azide!
    储存条件
    4 °C
  • 抗原
    Myc Tag
    Abstract
    Myc Tag 产品
    物质类
    Tag
    背景
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.  Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
    Synonyms: HRP
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