Biotin 抗体
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北京 101111
Quick Overview for Biotin 抗体 (ABIN349590)
抗原
片段
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 800-101-098
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Supplier
- Rockland
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原理
- Fab Biotin Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.
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产品特性
- Synonyms: Goat Fab Anti-Biotin Antibody, Goat Fab Fragment Anti-Biotin Antibody
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纯化方法
- Fab fragment of Anti-Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by papain digestion and chromatographic separation.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Immunogen Type: Other
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亚型
- IgG
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anti-Biotin antibody
WB, IHC, ELISA, FACS, IF, FM 宿主: 小鼠 Monoclonal 4C7-D5 unconjugated
anti-Biotin antibodyWB, IHC, FACS, IF, ISt, CM 宿主: 小鼠 Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyIHC, ISt 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibodyWB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Biotin antibody (DyLight 488)WB, FACS, IF, FM, FLISA, DB, MA 宿主: 山羊 Polyclonal DyLight 488
anti-Biotin antibody适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Biotin antibodyELISA, FACS 宿主: 小鼠 Monoclonal SB58c unconjugated
anti-Biotin antibodyELISA, FACS, IP, MS 宿主: 小鼠 Monoclonal 1D4-C5 unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Fab Anti-Biotin Antibody has been tested by ELISA and dot blot. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
ELISA Dilution: 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Biotin
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物质类
- Chemical
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背景
- Background: Fab Biotin antibody recognizes Biotin. Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.
抗原
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