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Phosphothreonine-PROLINE 抗体

The 小鼠 单克隆 anti-Phosphothreonine-PROLINE antibody (ABIN344517) specifically detects Phosphothreonine-PROLINE in WB, ELISA, IHC 和 IHC (p).
产品编号 ABIN344517
发货至: 中国
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Quick Overview for Phosphothreonine-PROLINE 抗体 (ABIN344517)

抗原

Phosphothreonine-PROLINE

宿主

  • 2
小鼠

克隆类型

  • 2
单克隆

标记

  • 2
非结合性

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    Detects phospho- threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context-independent fashion. The phosphospecific antibody does not recognize phospho-threonine in the absence of an adjacent proline. Does not react with phospho-tyrosine, but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e. g., phospho-Elk-1). Recognizes all species.

    纯化方法

    Protein A purified

    免疫原

    Synthetic phosphothreonine- proline-containing peptides (KLH coupled). Antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.

    Type of Immunogen: Synthetic peptide

    亚型

    IgM
  • 应用备注

    Approved: ELISA (1:1000), IHC, IHC-P, WB (1:5000)

    Usage: Western Blot: 1:5000. Immunohistochemistry (Paraffin): 1:50. ELISA: 1:1000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

    储存液

    Without preservative
  • 抗原

    Phosphothreonine-PROLINE

    别名

    Phospho-Threonine-Proline

    物质类

    Chemical
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