Phosphothreonine-PROLINE 抗体
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北京 101111
Quick Overview for Phosphothreonine-PROLINE 抗体 (ABIN344517)
抗原
宿主
克隆类型
标记
应用范围
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特异性
- Detects phospho- threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context-independent fashion. The phosphospecific antibody does not recognize phospho-threonine in the absence of an adjacent proline. Does not react with phospho-tyrosine, but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e. g., phospho-Elk-1). Recognizes all species.
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纯化方法
- Protein A purified
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免疫原
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Synthetic phosphothreonine- proline-containing peptides (KLH coupled). Antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.
Type of Immunogen: Synthetic peptide -
亚型
- IgM
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应用备注
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Approved: ELISA (1:1000), IHC, IHC-P, WB (1:5000)
Usage: Western Blot: 1:5000. Immunohistochemistry (Paraffin): 1:50. ELISA: 1:1000. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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储存液
- Without preservative
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- Phosphothreonine-PROLINE
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别名
- Phospho-Threonine-Proline
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物质类
- Chemical
抗原
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