West Nile Virus 抗体
Quick Overview for West Nile Virus 抗体 (ABIN340476)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes West Nile Virus NS2b subunit.
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纯化方法
- Protein G purified
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免疫原
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E. coli-derived rwnvNS3/NS2b.
Type of Immunogen: Recombinant protein -
亚型
- IgG3
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anti-West Nile Virus (WNV) (AA 73-86) antibody
WNV 适用: West Nile Virus (WNV), 人, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-West Nile Virus (WNV) (AA 1-135) antibodyWNV 适用: West Nile Virus (WNV) ELISA, WB 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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Approved: ELISA, IP, Neut, WB
Usage: Western Blot, Neutralization, Immunoprecipitation, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. Using a colorimetric detection system, the detection limit for rwnvNS3/NS2b complex is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Neutralization of west nile virus NS3 bioactivity: This antibody has been used to inhibit/neutralize the enzymatic activity of rwnvNS3/NS2b. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 min. The enzyme was then assayed with a peptide substrate, Ac-REKR-AFC. 50 % of the proteolytic activity was inhibited by the antibody at approximately 13 μg/mL (IC50) under conditions in which the enzyme was present at 10 μg/mL and the substrate concentration was 200uM. Considering the molecular masses of the enzyme (27kD) and the antibody (150kD), IC50 was achieved at approximately 1:4 Molar ratio of the antibody to the enzyme. Immunoprecipitation: This antibody was used at a concentration of 25 μg/mL to immunoprecipitate rwnvNS3/NS2b from conditioned cell culture medium. The recovered rwnvNS3/NS2b can be detected in Western blots using a polyclonal antibody. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. The detection limit for rwnvNS3/NS2b is approximately 1 ng/well. -
说明
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Target Species of Antibody: West Nile Virus
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution: Reconstitute, sterile TBS. If 1 mL of TBS is used, the antibody concentration will be 100 μg/mL.
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浓度
- Lot specific
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缓冲液
- Lyophilized from TBS, 5 % trehalose. Reconstitution: Reconstitute, sterile TBS. If 1 mL of TBS is used, the antibody concentration will be 100 μg/mL.
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- West Nile Virus (WNV)
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别名
- West Nile Virus
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物质类
- Virus
抗原
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