Ubiquitin 抗体
Our Local Distributor
北京 101111
Quick Overview for Ubiquitin 抗体 (ABIN340405)
抗原
See all Ubiquitin (Ub) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Recognizes ubiquitin, both conjugated and unconjugated. Reactivity has been shown in human, bovine, chicken, Drosophila and C. elegans.
-
纯化方法
- Purified
-
免疫原
-
Purified bovine ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin (KLH).
Type of Immunogen: Hapten -
亚型
- IgG1
-
-
anti-Ubiquitin (Ub) antibody
Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ub) (AA 1-229) antibodyUb 适用: 人, 大鼠, 小鼠, Cow, Pig, 犬, 马, 兔 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ub) (AA 1-229) antibodyUb 适用: 人, 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D2 unconjugated
anti-Ubiquitin (Ub) (acLys27) antibodyUb 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ub) antibodyUb 适用: 人, 大鼠, 小鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 5F1 unconjugated
anti-Ubiquitin (Ub) antibody (HRP)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal HRP
anti-Ubiquitin (Ub) antibody (FITC)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal FITC
anti-Ubiquitin (Ub) antibody (Biotin)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal Biotin
anti-Ubiquitin (Ub) antibody (PE)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal PE
anti-Ubiquitin (Ub) antibody (APC)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal APC
-
-
应用备注
-
Approved: IF (1:10), IHC, IHC-Fr, IHC-P, IP, WB (1:10)
Usage: Can be used for immunoassays, Western Blot, Immunofluorescence and Immunoprecipitation. Furthermore the antibody is useful for Immunohistology after heat-induced retrieval on both frozen and paraffin embedded sections. Immunohistology: 1:10. Immunofluorescence: 1:10. Western Blot: 1:10. For Western Blot it is recommended to use lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl, 20 mM Tris-HCl, 5 mM beta-mercaptoethanol, 1 mM PMSF ( pH 7.5)) for 30-60 minutes at 4?C, followed by extensive PBS washing. -
说明
-
Target Species of Antibody: Bovine
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, 0.02 % sodium azide, 0.1 % BSA.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
-
- Ubiquitin (Ub)
-
别名
- Ubiquitin
-
途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
抗原
-
