Phosphothreonine 抗体
Quick Overview for Phosphothreonine 抗体 (ABIN340104)
抗原
适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes Phosphothreonine, peptidyl-phosphothreonine, and threonine-phosphorylated proteins. Binding of Ly-pT-Gly-HRP (0.2 μg/100 μL/well) to an anti-phosphothreonine coated microplate (0.5 μg/100 μL/well) is inhibited by 95 % with the addition of 2-10 μg/mL anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1 mg/mL. Species cross-reactivity: species independent.
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纯化方法
- Immunoaffinity purified
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免疫原
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Keyhole Limpet Hemocyanin (KLH)- conjugated phosphothreonine conjugates and Phosvitin.
Type of Immunogen: Purified protein -
亚型
- IgG2a kappa
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anti-Phosphothreonine antibody
WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphothreonine antibody (FITC)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Phosphothreonine antibody (Biotin)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Phosphothreonine antibody (Atto 488)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-Phosphothreonine antibody (Atto 594)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Atto 594
anti-Phosphothreonine antibody (Atto 390)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Atto 390
anti-Phosphothreonine antibody (HRP)WB, ELISA, IP, IHC 宿主: 兔 Polyclonal HRP
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应用备注
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Approved: ELISA (0.5 μg/mL), IP, WB (2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot, and Immunoprecipitation. ELISA: 0.5 μg/mL (kinase assay). Western Blot: 2 μg/mL versus mouse brain lysate. Incubate at RT for 4 hours. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.1 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Phosphothreonine
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物质类
- Amino Acid
抗原
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