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Phosphothreonine 抗体

The 兔 多克隆 anti-Phosphothreonine antibody is suitable to detect Phosphothreonine. It has been validated for WB, IP 和 ELISA.
产品编号 ABIN340103
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Phosphothreonine 抗体 (ABIN340103)

抗原

Phosphothreonine

适用

请咨询

宿主

  • 17
  • 6

克隆类型

  • 16
  • 7
多克隆

标记

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This Phosphothreonine antibody is un-conjugated

应用范围

  • 23
  • 20
  • 16
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Western Blotting (WB), Immunoprecipitation (IP), ELISA
  • 特异性

    Recognizes phosphothreonine, peptidyl-phosphothreonine and threonine-phosphorylated proteins. Species cross-reactivity: species independent.

    纯化方法

    Immunoaffinity purified

    免疫原

    Phosphothreonine conjugated to KLH and phosvitin.

    Type of Immunogen: Hapten

    亚型

    IgG
  • 应用备注

    Approved: ELISA (0.5 μg/mL), IP, WB (2 μg/mL)

    Usage: Suitable for use in Western Blot, ELISA, and Immunoprecipitation. Western Blot: 2 μg/mL, Mouse brain lysate. Incubate at RT for 4 hrs. ELISA: 0.5 μg/mL (kinase assay). Binding of Ly-pT-Gly-HRP. (0.2 μg/100 μL/well) to an anti-phosphothreonine coated microplate (0.5 μg/100 μL/well) is inhibited by 95 % with the addition of 2-10 μg/mL anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1 mg/mL.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7, 0.01 % sodium azide, 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    Phosphothreonine

    物质类

    Amino Acid
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