Phosphothreonine 抗体
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- 抗原 See all Phosphothreonine products
- Phosphothreonine
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Phosphothreonine antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 特异性
- Recognizes phosphothreonine, peptidyl-phosphothreonine and threonine-phosphorylated proteins. Species cross-reactivity: species independent.
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Phosphothreonine conjugated to KLH and phosvitin.
Type of Immunogen: Hapten - 亚型
- IgG
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- 应用备注
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Approved: ELISA (0.5 μg/mL), IP, WB (2 μg/mL)
Usage: Suitable for use in Western Blot, ELISA, and Immunoprecipitation. Western Blot: 2 μg/mL, Mouse brain lysate. Incubate at RT for 4 hrs. ELISA: 0.5 μg/mL (kinase assay). Binding of Ly-pT-Gly-HRP. (0.2 μg/100 μL/well) to an anti-phosphothreonine coated microplate (0.5 μg/100 μL/well) is inhibited by 95 % with the addition of 2-10 μg/mL anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1 mg/mL. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7, 0.01 % sodium azide, 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 抗原
- Phosphothreonine
- Abstract
- Phosphothreonine 产品
- 物质类
- Amino Acid
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