Phosphoserine 抗体
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北京 101111
Quick Overview for Phosphoserine 抗体 (ABIN340085)
抗原
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes phosphoserine, peptidyl-phosphoserine and serine-phosphorylated proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein. Species cross-reactivity: All species.
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纯化方法
- Immunoaffinity purified
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免疫原
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Phosphoserine conjugated with KLH.
Type of Immunogen: Hapten -
亚型
- IgG
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anti-Phosphoserine antibody
适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 7A3 unconjugated
anti-Phosphoserine (phosphorylated) antibodyWB 宿主: 小鼠 Monoclonal 5B12 unconjugated
anti-Phosphoserine antibodyWB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoserine antibodyWB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphoserine antibody (FITC)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Phosphoserine antibody (Biotin)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Phosphoserine antibody (Atto 390)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 390
anti-Phosphoserine antibody (Atto 488)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-Phosphoserine antibody (Atto 594)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 594
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应用备注
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Approved: ELISA (1:250 - 1:1000), IHC (1:50), IP, WB
Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ELISA. Western Blot (tissue lysates): 50-100 μg/lane total protein, antibody dilution can be from 1:250-1:1000, overloading will show solid exposed lanes. Dilute the primary antibody until multiple phosphorylated bands will appear. If using purified proteins dilutions from 1:50-1:1000 will need to be tried. At 1:300 dilution the antibody normally detects 10 ng of protein with 2 hour incubations and enhanced HRP detection. Blocking of the blot should be with non-phosphoserine containing compounds such as 1-5 % BSA in TBS that has been filtered through a 0.45um. Milk or casein is not recommended because of the presence of phospho-serine in these solutions. Buffers should be Tris based so that phosphate ion interference is minimized. Immunoprecipitation (tissue extracts): 10-20 μg/mg protein. Note that this will immunoprecipitate all phosphoserine proteins in the extracts. If using purified protein exacts containing only the protein of interest, antibody amounts should be decreased to 5 μg/500 μL of extracts. ELISA (kinase assay): 1:250-1:500. Immunohistochemistry: 1:50. Detects all phosphoserines, and any phosphorylated serine protein or peptide can be used to block antibody staining. Typically, a10M excess of peptide is used in Tris based buffers. Positive control: NIH 3T3 cells (+/- TPA), K562 cells and EGF-stimulated A431 cells. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 50 % glycerol.
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注意事项
- Avoid repeated freezing and thawing
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储存条件
- -20 °C
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储存方法
- For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.
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- Phosphoserine
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物质类
- Amino Acid
抗原
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