Luciferase 抗体
Quick Overview for Luciferase 抗体 (ABIN339711)
抗原
See all Luciferase 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- This antibody specifically reacts with luciferase protein, a reporter used to quantify expression levels from promoters in tissue culture and in vivo. Reactivity was confirmed via Western Blot analysis on the following samples containing added purified luciferase protein: mouse heart, liver, kidney, and cortex tissues. There was no cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts. In IHC (frozen tissue) and Immunofluorescence applications, our antibody was able to detect tissue specific expression of Luciferase protein in Drosophila embryos using the well-characterized Gal4-UAS system with two different Gal4 drivers.
-
纯化方法
- Protein A purified
-
免疫原
-
Luciferase protein from Photinus pyralis.
Type of Immunogen: Purified protein -
亚型
- IgG1
-
-
anti-Luciferase antibody
适用: Firefly ELISA, IF (p), IF (cc), IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, IF 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, ELISA, IHC, FM 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB 宿主: 小鼠 Monoclonal Luci17 unconjugated
anti-Luciferase (C-Term) antibody适用: Photinus pyralis WB 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase (N-Term) antibody适用: Photinus pyralis WB, IHC 宿主: 小鼠 Monoclonal Luci17 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, IP 宿主: 小鼠 Monoclonal 2D4 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, ELISA 宿主: 山羊 Polyclonal unconjugated
-
-
应用备注
-
Approved: IF (10 - 30 μg/mL), IHC, IHC-Fr, WB (1 - 3 μg/mL)
Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Western Blot: 1-3 μg/mL. Observed in mouse heart, liver, kidney, and cortex tissues containing added purified luciferase protein. Immunohistochemistry (Frozen Tissue): 15-50 μg/mL. Detects tissue specific expression of Luciferase protein in Drosophila embryos using Gal4-UAS system with two different Gal4 drivers. Immunofluorescence: 10-30 μg/mL. -
说明
-
Target Species of Antibody: Photinus pyralis
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, pH 7.4, 0.1 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid freeze-thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
-
- Luciferase
-
别名
- Firefly Luciferase
-
背景
- Synonyms: Firefly Luciferase, PPLUC
-
UniProt
- P08659
抗原
-
