AKT Substrate (pSer), (pThr) 抗体
This anti- antibody is a 兔 多克隆 antibody detecting in ELISA, WB, IHC (p) 和 IP. Suitable for .
Quick Overview for AKT Substrate (pSer), (pThr) 抗体 (ABIN337521)
抗原
AKT Substrate
适用
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宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
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抗原表位
- pSer, pThr
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特异性
- Preferentially recognizes peptides and proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3, in a manner largely independent of other surrounding amino acids. Some cross-reactivity is observed for peptides that contain phospho-Ser/Thr preceded by Arg/Lys at positions -3 and -2. No cross-reactivity is observed with the corresponding non-phosphorylated sequences or with other phospho-Ser/Thr-containing motifs. By ELISA the antibody recognizes a wide range of phosphorylated Akt substrate peptides and by 2D gel Western blot analysis it recognizes a large number of presumptive Akt substrate proteins.
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纯化方法
- Immunoaffinity Chromatography
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免疫原
- Synthetic phospho- Akt substrate peptides.
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亚型
- IgG
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应用备注
- ELISA (1:1000), IHC-P, IP (1:20), WB (1:1000)
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, BSA, 50 % glycerol.
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注意事项
- avoid freeze thaw cycles. Store undiluted.
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储存条件
- 4 °C/-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
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- AKT Substrate
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物质类
- Chemical
抗原
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