Transglutaminase 2 抗体
Quick Overview for Transglutaminase 2 抗体 (ABIN337398)
抗原
See all Transglutaminase 2 (TGM2) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes Transglutaminase II. Species cross-reactivity: Human, mouse, rat.
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纯化方法
- Protein A purified
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免疫原
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Recombinant full length human TGase II (77-85kD). Cellular Localization: Cytoplasm, cell membrane, and extracellular matrix.
Type of Immunogen: Recombinant protein -
亚型
- IgG
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应用备注
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Approved: IHC, IHC-P (5 μg/mL), IP (10 μg/tst), WB (5 - 10 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation (Native verified): 10 μg/mg protein lysate. Use Protein A. Western Blot: 5-10 μg/mL for 2hrs at RT. Positive control: HUVEC cells. Breast carcinoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4.
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储存液
- Azide free
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注意事项
- avoid freeze thaw cycles. Store undiluted.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
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- Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))
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别名
- TGM2 / Transglutaminase 2
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背景
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Name/Gene ID: TGM2
Synonyms: TGM2, G-ALPHA-h, GNAH, TG(C), Transglutaminase 2, Transglutaminase C, TG2, TGase H, TGase-2, TGase-H, Transglutaminase H, TGase C, TGC, TTG, C polypeptide, Tgase II, Tissue transglutaminase, Transglutaminase-2 -
基因ID
- 7052
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UniProt
- P21980
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途径
- Tube Formation, Thromboxane A2 Receptor Signaling
抗原
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