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Complement C2 抗体

This anti-Complement C2 antibody is a 小鼠 单克隆 antibody detecting Complement C2 in WB, ELISA, IHC 和 IHC (p). Suitable for 人.
产品编号 ABIN337358
发货至: 中国

Quick Overview for Complement C2 抗体 (ABIN337358)

抗原

See all Complement C2 抗体
Complement C2

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Complement C2 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 品牌

    IHC-plus™

    特异性

    Recognizes C2 from human plasma/serum. Epitope specificity differs from that of LS-C22277 and LS-C22278 as determined by inhibition ELISA. Reacts strongly with C2. Strong reaction is seen with human serum/plasma and no reaction is seen with serum from patients deficient in C2, when tested in sandwich ELISA with a polyclonal antibody against C2. In ELISA, reaction is also seen with C2 directly coated onto the microtiter well.

    纯化方法

    Protein A/G purified

    免疫原

    Complement component C2 isolated from human plasma.

    Type of Immunogen: Purified protein

    亚型

    IgG1 kappa
  • 应用备注

    Approved: ELISA (1:2000), IHC, IHC-P (10 μg/mL), WB (1:125)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: After SDS-PAGE, this antibody reacts with C2 and a subfraction of C2 believed to be C2b, both under reducing and non-reducing conditions.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 15 mM sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原

    Complement C2

    背景

    Name/Gene ID: C2
    Subfamily: Serine S1
    Family: Protease

    Synonyms: C2, C2b, C3/C5 convertase, Complement component C2, Complement C2, Complement component 2, CO2

    基因ID

    717

    UniProt

    P06681
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