FHL1 抗体 (AA 261-272)
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Quick Overview for FHL1 抗体 (AA 261-272) (ABIN337221)
抗原
See all FHL1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 261-272
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特异性
- Human FHL1. This antibody is expected to recognise isoforms 2( NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.
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预测反应
- Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%) Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Sheep, Elephant, Bovine, Dog, Horse, Rabbit, Pig, Xenopus (92%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide CNKRFVFHNEQVY from an internal region of human FHL1 (NP_001440.1, NP_001153171.1). Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%), Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Sheep, Elephant, Bovine, Dog, Horse, Rabbit, Pig, Xenopus (92%).
Type of Immunogen: Synthetic peptide
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应用备注
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Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.05 - 0.3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze-thaw cycles.
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- FHL1 (Four and A Half LIM Domains 1 (FHL1))
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别名
- SLIM / FHL1
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背景
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Name/Gene ID: FHL1
Synonyms: FHL1, BA535K18.1, FHL1B, FHL1A, FLH1A, Four and a half LIM domains 1, FHL-1, KYOT, Skeletal muscle LIM-protein 1, SLIM-1, Skeletal muscle LIM-protein1, SLIMMER, XMPMA, KYO-T, LIM protein SLIMMER, SLIM, SLIM1 -
基因ID
- 2273
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NCBI登录号
- NP_001440, NP_001153171
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UniProt
- Q13642
抗原
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