Tyrosine Hydroxylase 抗体 (pSer40)
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Quick Overview for Tyrosine Hydroxylase 抗体 (pSer40) (ABIN337188)
抗原
See all Tyrosine Hydroxylase (TH) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer40
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特异性
- Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.
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纯化方法
- Immunoaffinity purified
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免疫原
- Phosphopeptide corresponding to amino acid residues surrounding the phosphoSer40 of rat tyrosine hydroxylase.
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应用备注
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Approved: IF (1:1000), IHC, IHC-Fr (1:1000), IHC-P (5 μg/mL), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
说明
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Target Species of Antibody: Rat
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.
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储存条件
- -20 °C,-80 °C
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储存方法
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Short term: -20°C
Long term: -80°C.
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- Tyrosine Hydroxylase (TH)
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别名
- TH / Tyrosine Hydroxylase
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背景
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Name/Gene ID: TH
Family: Monooxygenase
Synonyms: TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase -
基因ID
- 7054
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UniProt
- P07101
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途径
- Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
抗原
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