Pyruvate Kinase, Muscle (PKM) 抗体
Quick Overview for Pyruvate Kinase, Muscle (PKM) 抗体 (ABIN337171)
抗原
See all Pyruvate Kinase, Muscle (PKM) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes endogenous levels of total human PKM2 protein at 60kD. Species cross-reactivity: monkey, mouse and rat.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide derived from the sequence of human PKM2 (KLH). Molecular Weight: 60kD.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: IHC, IHC-Fr (1:100), IHC-P (1:100), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Pyruvate Kinase, Muscle (PKM)
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别名
- PKM / Pyruvate Kinase, Muscle
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背景
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Name/Gene ID: PKM
Synonyms: PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1 -
分子量
- 60kDa
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基因ID
- 5315
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UniProt
- P14618
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途径
- Warburg Effect
抗原
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