P4HB 抗体
Our Local Distributor
北京 101111
Quick Overview for P4HB 抗体 (ABIN337100)
抗原
See all P4HB 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Recognizes endogenous levels of total human PDI protein. Species cross-reactivity: monkey, mouse and rat.
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
Synthetic peptide derived from the sequence around Pro329 of human PDI (KLH).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
-
-
-
-
应用备注
-
Approved: ICC (1:50), IF (1:50), IHC, IHC-P (1:50), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Immunofluorescence (IF-IC): 1:25. Requires methanol permeabilization. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- Supplied as a liquid in 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.
-
注意事项
- Avoid repeat freeze-thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
-
- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
-
别名
- PDI / P4HB
-
背景
-
Name/Gene ID: P4HB
Synonyms: P4HB, ERBA2L, DSI, GIT, p4HB, p4Hbeta, PDIA1, PHDB, PO4DB, PROHB, Protocollagen hydroxylase, Protein disulfide-isomerase, PO4HB, PDI -
基因ID
- 5034
-
UniProt
- P07237
-
途径
- Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
抗原
-