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P4HB 抗体

The 兔 多克隆 anti-P4HB antibody is suitable to detect P4HB in samples from 人. It has been validated for WB, IHC, ICC, IF 和 IHC (p).
产品编号 ABIN337100
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for P4HB 抗体 (ABIN337100)

抗原

See all P4HB 抗体
P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

适用

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宿主

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克隆类型

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多克隆

标记

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This P4HB antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    Recognizes endogenous levels of total human PDI protein. Species cross-reactivity: monkey, mouse and rat.

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide derived from the sequence around Pro329 of human PDI (KLH).

    Type of Immunogen: Synthetic peptide

    亚型

    IgG
  • 应用备注

    Approved: ICC (1:50), IF (1:50), IHC, IHC-P (1:50), WB (1:1000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Immunofluorescence (IF-IC): 1:25. Requires methanol permeabilization.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Supplied as a liquid in 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

    别名

    PDI / P4HB

    背景

    Name/Gene ID: P4HB

    Synonyms: P4HB, ERBA2L, DSI, GIT, p4HB, p4Hbeta, PDIA1, PHDB, PO4DB, PROHB, Protocollagen hydroxylase, Protein disulfide-isomerase, PO4HB, PDI

    基因ID

    5034

    UniProt

    P07237

    途径

    Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
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