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OB Cadherin 抗体 (Intracellular)

This anti-OB Cadherin antibody is a 兔 多克隆 antibody detecting OB Cadherin in WB, ELISA, IHC, IHC (fro) 和 IHC (p). Suitable for 人 和 小鼠.
产品编号 ABIN337036
发货至: 中国

Quick Overview for OB Cadherin 抗体 (Intracellular) (ABIN337036)

抗原

See all OB Cadherin (CDH11) 抗体
OB Cadherin (CDH11) (Cadherin 11 (CDH11))

适用

  • 85
  • 40
  • 21
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  • 2
人, 小鼠

宿主

  • 54
  • 28
  • 4
  • 2

克隆类型

  • 59
  • 29
多克隆

标记

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  • 1
  • 1
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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This OB Cadherin antibody is un-conjugated

应用范围

  • 63
  • 38
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  • 12
  • 11
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  • 6
  • 3
  • 2
  • 1
  • 1
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

    • 14
    • 11
    • 8
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    • 3
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    • 3
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    • 3
    • 1
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    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    Intracellular

    品牌

    IHC-plus™

    特异性

    Recognizes human Cadherin 11 at ~110kD. An additional band at ~130kD (presumably the Cadherin 11 precursor) may also be observed. Some crossreactivity with other Cadherins is observed in approximately the following order: Cadherin11>>R-Cadherin>Cadherin 8>>N-Cadherin=P-Cadherin. No crossreactivity with Cadherin 6 or E-Cadherin has been observed.

    纯化方法

    Immunoaffinity purified

    免疫原

    Recombinant protein derived from the intracellular domain of the human Cadherin 11 protein.

    Type of Immunogen: Recombinant protein

    亚型

    IgG
  • 应用备注

    Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1 - 2 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: MDA. MB231 cells (human) and mouse L fibroblast lysates.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.09 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    OB Cadherin (CDH11) (Cadherin 11 (CDH11))

    别名

    CDH11 / Cadherin 11

    背景

    Name/Gene ID: CDH11
    Family: Cadherin

    Synonyms: CDH11, CAD11, Cadherin 11, Cadherin-11, CDHOB, OSF-4, Osteoblast cadherin, OB, OB-cadherin, Ventral neural cadherin, Vnc

    基因ID

    1009

    UniProt

    P55287
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