S1PR3 抗体 (AA 12-25)
Quick Overview for S1PR3 抗体 (AA 12-25) (ABIN337001)
抗原
See all S1PR3 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 12-25
-
品牌
- IHC-plus™
-
特异性
- Recognizes human EDG 3 receptor at ~47kD in human cerebral cortex. Liver and heart tissues display 2-3 bands between 40-50kD, possibly due to different degrees of glycosylation.
-
预测反应
- Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon (93%) Marmoset (86%).
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
Synthetic peptide corresponding to aa12-25 [VRGNETLREHYQYV] of human EDG 3 protein. Species sequence Homology: Mouse, rat: 79% (11/14). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon (93%), Marmoset (86%).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
-
-
-
-
应用备注
-
Approved: ICC, IHC, IHC-P (10 μg/mL), WB (3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- TBS, pH 7.4, 5 mg/mL BSA
-
注意事项
- Avoid repeat freeze-thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
-
- S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))
-
别名
- SIPR3 / EDG3 / S1P3
-
背景
-
Name/Gene ID: S1PR3
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR
Synonyms: S1PR3, EDG-3, EDG3, LPB3, S1P receptor EDG3, S1P3, S1P receptor Edg-3, S1P receptor 3 -
基因ID
- 1903
-
UniProt
- Q99500
抗原
-