Fibronectin 抗体
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北京 101111
Quick Overview for Fibronectin 抗体 (ABIN336929)
抗原
See all Fibronectin 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts with human fibronectin in liver, tonsil, skin and kidney. Molecular Weight: Non reduced 400-440 kDa, reduced 200-220 kDa.
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免疫原
- Purified human Fibronectin from human plasma
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应用备注
- Immunohistochemistry (IHC) 1:100-1:400 for 30 minute at room temp. using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears require antigen retriever, boiling tissue in 10 mM citrate buffer, pH 6.0 for 10-20 minute, followed by cooling at room temp. for 20 minutes. The optimum dilution should be determined by the individual lab. Positive Control: Human kidney. Cellular Localization: Connective tissue matrix and basement membrane.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Immunized serum in10 mM PO4, 150 mM NaCl, pH 7.4, 0.05% sodium azide. HostRabbit ReagentConc. antibody
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- Fibronectin
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背景
- Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and extracellular matrix. Fibronectin is involved in cell adhesion and migration process including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They also occur as dimmer of two 200 kDa subunits. Fibronectin has been implicated in carcinoma development in lung cancer. Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents.
抗原
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