Cofilin 抗体 (N-Term)
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Quick Overview for Cofilin 抗体 (N-Term) (ABIN336102)
抗原
See all Cofilin (CFL1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-166, N-Term
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特异性
- The antibody recognizes Human Cofilin-1. Other species not tested.
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产品特性
- Synonyms: CFL1, CFL, p18, Cofilin non-muscle isoform, 18 kDa phosphoprotein
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纯化方法
- Protein-G affinity chromatography
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免疫原
- Recombinant human Cofilin-1 (aa 1-166) purified from E. coli
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亚型
- IgG1
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应用备注
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ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This Cofilin 1 antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, containing 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Cofilin (CFL1) (Cofilin 1 (CFL1))
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别名
- Cofilin-1
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背景
- Cofilin is a family of actin-binding proteins which disassembles actin filaments. It is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH -dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.Synonyms: 18 kDa phosphoprotein, CFL, CFL1, Cofilin non-muscle isoform, p18
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基因ID
- 1072
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UniProt
- P23528
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途径
- Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events
抗原
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