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Vimentin 抗体

VIM 适用: 人, 大鼠, Pig, 犬, 山羊, Hamster WB, IHC, FACS, ICC, IHC (fro) 宿主: 小鼠 Monoclonal RV203 unconjugated
产品编号 ABIN335381
发货至: 中国
  • 抗原 See all Vimentin (VIM) 抗体
    Vimentin (VIM)
    适用
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    人, 大鼠, Pig, 犬, 山羊, Hamster
    宿主
    • 190
    • 128
    • 9
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    • 2
    小鼠
    克隆类型
    • 198
    • 144
    单克隆
    标记
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    This Vimentin antibody is un-conjugated
    应用范围
    • 281
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    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    Human, rat, goat, canine, hamster and swine.
    纯化方法
    Purified
    免疫原
    RV203 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
    克隆位点
    RV203
    亚型
    IgG1
    Top Product
    Discover our top product VIM Primary Antibody
  • 应用备注
    RV203 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV203 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
    限制
    仅限研究用
  • 储存条件
    4 °C
  • Schaart, Pieper, Kuijpers, Bloemendal, Ramaekers: "Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins." in: Differentiation; research in biological diversity, Vol. 46, Issue 2, pp. 105-15, (1991) (PubMed).

  • 抗原
    Vimentin (VIM)
    别名
    Vimentin (VIM 产品)
    别名
    CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody
    背景
    Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
    途径
    Caspase Cascade in Apoptosis
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