160 kDa Neurofilament 抗体
适用: 人, Hamster, 猴, 非洲爪蟾, 豚鼠, 兔
WB, IHC, IHC (fro), IHC (p)
宿主: 小鼠
Monoclonal
RNF406
unconjugated
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- 抗原
- 160 kDa Neurofilament
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适用
- 人, Hamster, 猴, 非洲爪蟾, 豚鼠, 兔
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Human, rabbit, hamster, monkey, guinea pig and Xenopus.
- 纯化方法
- Purified
- 免疫原
- RNF406 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a neurofilament preparation of calf brain tissue.
- 克隆位点
- RNF406
- 亚型
- IgG1
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- 应用备注
- RNF406 reacts exclusively with the phosphorylated isoform of the160 kD neurofilament protein. RNF406 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:50 - 1:100 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原
- 160 kDa Neurofilament
- 别名
- Neurofilament 160 kDa
- 背景
- Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.
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