Cytokeratin 13 抗体
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北京 101111
Quick Overview for Cytokeratin 13 抗体 (ABIN335313)
抗原
See all Cytokeratin 13 (KRT13) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse anti Cytokeratin 13
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特异性
- Ks13.1 reacts exclusively with Cytokeratin 13 which is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stRatified epithelia. As a result the antibody also reacts with neoplasms derived from these epithelia.
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产品特性
- Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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纯化方法
- culture supernatant
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免疫原
- Ks13.1 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a Cytokeratin preparation extracted from Human esophagus.
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亚型
- IgG1
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anti-Keratin 13 (KRT13) (N-Term) antibody
KRT13 适用: 大鼠, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 13 (KRT13) (C-Term) antibodyKRT13 适用: 大鼠, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 13 (KRT13) (AA 104-458) antibodyKRT13 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 7D7B3 unconjugated
anti-Keratin 13 (KRT13) antibodyKRT13 适用: 人 WB, IF, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 13 (KRT13) (AA 104-403) antibodyKRT13 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 13 (KRT13) (AA 143-295) antibodyKRT13 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3G4 unconjugated
anti-Keratin 13 (KRT13) (AA 269-458) antibodyKRT13 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 13 (KRT13) antibodyKRT13 适用: 人 WB, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
anti-Keratin 13 (KRT13) antibodyKRT13 适用: 人 IHC, IF, FACS 宿主: 小鼠 Monoclonal KRT13-2659 unconjugated
anti-Keratin 13 (KRT13) (AA 106-138), (N-Term) antibodyKRT13 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB18903 unconjugated
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应用备注
- Ks13.1 is suitable for immunoblotting and immunohistochemistry on frozen tissues and paraffin embedded tissue after protease pretreatment. Optimal antibody dilution should be determined by titration.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Each vial contains 1 mL of culture supernatant of monoclonal antibody containing 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
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: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, (1996) (PubMed).
: "Immunohistochemical studies on uterine tumors. I. Invasive squamous cell carcinomas of the cervix and their precursors." in: Pathology, research and practice, Vol. 187, Issue 1, pp. 36-43, (1991) (PubMed).
: "Cytokeratins in normal and malignant transitional epithelium. Maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines." in: The American journal of pathology, Vol. 132, Issue 1, pp. 123-44, (1988) (PubMed).
: "Patterns of expression of cytoskeletal proteins in human thyroid gland and thyroid carcinomas." in: Differentiation; research in biological diversity, Vol. 35, Issue 1, pp. 53-71, (1988) (PubMed).
: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, (1983) (PubMed).
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- Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))
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别名
- Cytokeratin 13 / Keratin K13
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UniProt
- P13646
抗原
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