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Ascorbate Oxidase 抗体

The 兔 多克隆 anti-Ascorbate Oxidase antibody is suitable to detect Ascorbate Oxidase in samples from Cucurbita pepo. It has been validated for ELISA, WB 和 IHC.
产品编号 ABIN334592
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Ascorbate Oxidase 抗体 (ABIN334592)

抗原

Ascorbate Oxidase (AO)

适用

  • 13
  • 2
Cucurbita pepo

宿主

  • 15

克隆类型

  • 15
多克隆

标记

  • 6
  • 4
  • 3
  • 2
This Ascorbate Oxidase antibody is un-conjugated

应用范围

  • 13
  • 11
  • 4
  • 2
  • 2
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
  • 原理

    Anti-AO | ascorbate oxidase

    交叉反应 (详细)

    Cucurbita sp.
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known

    预测反应

    Glycne max,Oryza sativa

    免疫原

    Ascorbate oxidase purified from Cucurbita sp.

    亚型

    IgG
  • 应用备注

    1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 0.5 mL of sterile water

    缓冲液

    Total IgG in PBS pH 7.2.

    注意事项

    Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

    储存条件

    4 °C,-20 °C

    储存方法

    Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • 抗原

    Ascorbate Oxidase (AO)

    背景

    Background: Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90 % of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

    Additional information: IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography,

    分子量

    61 | 45 kDa (Cucurbita pepo)
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