Ascorbate Oxidase 抗体 (Biotin)
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北京 101111
Quick Overview for Ascorbate Oxidase 抗体 (Biotin) (ABIN334591)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-AO | ascorbate oxidase, Biotin conjugated
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交叉反应 （详细）
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Cucurbita sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Glycne max,Oryza sativa
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纯化方法
- Purified IgG
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免疫原
- ascorbate oxidase purified from Cucurbita sp.
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亚型
- IgG
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anti-Ascorbate Oxidase (AO) (AA 31-579) antibody
AO 适用: Pumpkin ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)
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说明
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IgG concentration is 10 mg/ml. Biotin/IgG protein molar ratio is approximately 5.6. No foreign proteins are added.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 1 mL of sterile water, Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution, If necessary, centrifuge to remove any particulates, Prepare fresh working dilution daily
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缓冲液
- Purified IgG in PBS pH 7.2.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- 4 °C,-20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.
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- Ascorbate Oxidase (AO)
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背景
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Background: Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90 % of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
Additional information: IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography, N-Hydroxysuccinimidobiotin is used for labelling of antibody
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分子量
- 61 | 45 kDa (Cucurbita pepo)
抗原
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