Amylase beta 抗体 (Biotin)
This anti-Amylase beta antibody is a 兔 多克隆 antibody detecting Amylase beta in WB, ELISA, IF 和 IHC. Suitable for Potato.
Quick Overview for Amylase beta 抗体 (Biotin) (ABIN334590)
抗原
Amylase beta (amyB)
适用
Potato
宿主
兔
克隆类型
多克隆
标记
This Amylase beta antibody is conjugated to Biotin
应用范围
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
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交叉反应 （详细）
- Not reactive in: n.a.
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预测反应
- dicots including: Arabidopsis thaliana, Glycine max, Ricinus communis, Vitis vinifera, trees: Populus trichocarpa, Physcomitrella patens
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产品特性
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Expected / apparent Molecular Weight of the Antigene: 60 kDa
Detection limit is 12.25 pg, 7 fmol, linear range of logit/log plot is 0.02-10 pmol,midrange (B/ B o =50 %) 44.28 pg, 253 fmol, unspecific binding 2.5%. -
纯化方法
- affinity purified
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免疫原
- Beta amylase isolated and purified from sweet potato Q94EU9
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亚型
- IgG
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应用备注
- 1: 1000 to 1: 4000 for techniques listed above
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说明
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antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution please add 1 mL of sterile destilled water.
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浓度
- 10 mg/mL
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缓冲液
- PBS labelled with biotin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
储存条件
- -20 °C
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储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Amylase beta (amyB)
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别名
- Beta-amylase
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背景
- Beta amylase (EC 3.2.1.2.) catalyzes the hydrolysis of the second alfa-1,4 glycosidic bond. Alternative names 1,4-alfa-D-glucan maltohydrolase, glycogenase,saccharogen. amylase).
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分子量
- 60 kDa
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UniProt
- Q94EU9
抗原
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