50S Ribosomal Protein L30 抗体
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北京 101111
Quick Overview for 50S Ribosomal Protein L30 抗体 (ABIN7676204)
抗原
适用
宿主
克隆类型
应用范围
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原理
- Anti-L7/L12 | Ribosomal protein
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交叉反应 （详细）
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Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
纯化方法
- Serum
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免疫原
- Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece
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应用备注
- 1: 10 (IF), 1 : 1000 (WB)
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说明
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Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- 50S Ribosomal Protein L30
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别名
- 50S ribosomal protein L30 (L-30)
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背景
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Background: Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463
Additional information: Name of this antibody has been changed from L-30 | 50S ribosomal protein L30 to L7/L12 based on the following reference: Randolph-Anderson et al. (1989). Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol. 1989 Jul,29(1):68-88.
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分子量
- 11.9 kDa (Chlamydomonas reinhardtii), 15.7 kDa (spinach)
抗原
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