TOP2 抗体 (C-Term)
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Quick Overview for TOP2 抗体 (C-Term) (ABIN334538)
抗原
See all TOP2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Anti-TOP2 | DNA topoisomerase II
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特异性
- Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis cells the antibody detects a 170 kDa protein exclusively in the nulear fraction.
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交叉反应 （详细）
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Arabidopsis thaliana, Vicia faba
Not reactive in: Nicotiana tabacum -
预测反应
- Brassica rapa,Chlamydomonas reinhardtii,Chlorella vulgaris,Citrus clementina,Glycine max,Hordeum vulgare,Medicago truncatula,Oryza sativa,Ostreococcus tauri,Panicum italicum,Phaseolus vulgaris,Physcomitrium patens,Pinus sitcHensis,Populus trichocarpa,Solanum tuberosum,Sorghum bicolor,Triticum aestivum,Vitis vinifera,Volvox Caterii
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纯化方法
- Serum
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免疫原
- The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890
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anti-DNA Topoisomerase II (TOP2) (AA 923-1148) antibody
TOP2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA Topoisomerase II (TOP2) (AA 1079-1328) antibodyTOP2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA Topoisomerase II (TOP2) (AA 749-987) antibodyTOP2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 : 500 (IL), 1 : 2000 (WB)
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说明
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Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." in: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).
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: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." in: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).
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- TOP2 (DNA Topoisomerase II (TOP2))
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别名
- TOP2
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背景
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Background: Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II
Additional information: Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis thaliana cells the antibody detects a 170 kDa protein exclusively in the nulear fraction (see picture).
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分子量
- 164 | 170 kDa (Arabidopsis thaliana)
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基因ID
- 821972
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UniProt
- P30182
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途径
- Mitotic G1-G1/S Phases
抗原
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