COXII 抗体
There are 4+ publications for this product available. The 兔 多克隆 anti-COXII antibody is suitable to detect COXII in samples from Cabbage, Physcomytrium Patens, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Arabidopsis thaliana, durum wheat, Zea mays, Vicia faba, Triticum aestivum, Rape, Quercus rubra, Picea abies, Nicotiana tabacum, Lilium longiflorum (Trumpet lily), Eriophorum vaginatum, Cucumis sativus, Cucumis melo, Chickpea, Betula nana, Barley 和 Actinidia chinensis. It has been validated for WB 和 BN PAGE.
1 image
(4 references)
产品编号 ABIN334535
发货至:
中国
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Quick Overview for COXII 抗体 (ABIN334535)
抗原
COXII
(Cytochrome Oxidase Subunit II (COXII))
适用
Cabbage, Physcomytrium Patens, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Arabidopsis thaliana, durum wheat, Zea mays, Vicia faba, Triticum aestivum, Rape, Quercus rubra, Picea abies, Nicotiana tabacum, Lilium longiflorum (Trumpet lily), Eriophorum vaginatum, Cucumis sativus, Cucumis melo, Chickpea, Betula nana, Barley, Actinidia chinensis
宿主
兔
克隆类型
多克隆
标记
This COXII antibody is un-conjugated
应用范围
Western Blotting (WB), Blue-native PAGE (BN PAGE)
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原理
- Anti-COXII | Plant Cytochrome oxidase subunit II (affinity purified)
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特异性
- Arabidopsis thaliana (leaf extract and isolated mitochondria).
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交叉反应 （详细）
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Actinidia chinensis, Arabidopsis thaliana (leaf extract and isolated mitochondria), Betula nana, Brassica napus, Brassica oleracea, Cicer aretinum, Cucumis melo, Cucumis sativus, Erophorum vaginatum, Hordeum vulgare, Lilium longifolorum, Nicotiana tabacum, Picea abies, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica, Physcomitrium patens, Triticum aestivum, Triticum durum Desf., Zea mays, Vicia faba, Quercus rubra
Not reactive in: Mangifera indica, Saccharina japonica -
预测反应
- Cucumis melo,Glycine max,Gossypium hirsutum,Neurachne alopecuroidea,Neurachne minor,Neurachne muelleri,Oryza sativa,Physcomitrium patens,Pisum sativum,Triticum aestivum,Vigna radiata
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160
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应用备注
- 1 : 1000 (BN-PAGE), 1 : 1000 (WB)
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说明
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antibody detects COXII protein in total leaf extracts and isolated mitochondriaBlue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).
: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).
: "The plasma membrane proteome of maize roots grown under low and high iron conditions." in: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).
: "In the heat of the night - alternative pathway respiration drives thermogenesis in Philodendron bipinnatifidum." in: The New phytologist, (2010) (PubMed).
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: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).
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- COXII (Cytochrome Oxidase Subunit II (COXII))
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别名
- COXII
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背景
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Background: Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2
Additional information: Cellular [compartment marker] of mitochondrial inner membrane
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分子量
- 29.4 | 30 kDa (for Arabidopsis thaliana)
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基因ID
- 3989052
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UniProt
- P04373, Q1XGA9
抗原
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