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HNF1B 抗体 (Internal Region)

This anti-HNF1B antibody is a 山羊 多克隆 antibody detecting HNF1B in WB, ELISA 和 ICC. Suitable for 人 和 小鼠. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN334505
发货至: 中国

Quick Overview for HNF1B 抗体 (Internal Region) (ABIN334505)

抗原

See all HNF1B 抗体
HNF1B (HNF1 Homeobox B (HNF1B))

适用

  • 58
  • 25
  • 22
  • 6
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  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
人, 小鼠

宿主

  • 51
  • 10
山羊

克隆类型

  • 50
  • 10
  • 1
多克隆

标记

  • 46
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
This HNF1B antibody is un-conjugated

应用范围

  • 48
  • 25
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  • 5
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

质量等级

Verified
  • 抗原表位

    • 8
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    原理

    TCF2 / VHNF1

    序列

    QAYDRQKNPS KEER

    特异性

    This antibody is expected to recognize both reported isoforms (NP_000449.1, NP_001159395.1). This product may cross-react with HNF1A.

    交叉反应

    Cow, 犬, 人, 小鼠, Pig, 大鼠, 斑马鱼

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-QAYDRQKNPSKEER, from the internal region of the protein sequence according to NP_000449.1, NP_001159395.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 60 kDa band observed in Human Liver lysates (calculated MW of 61.3 kDa according to NP_000449.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. An additional band of unknown identity was also consistently ob

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunocytochemistry: A customer found positive results in ICC on Human primitive gut tube from Human embryonic stem cells.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Arenas, Hervias, Uriz, Joplin, Prieto, Medina: "Combination of ursodeoxycholic acid and glucocorticoids upregulates the AE2 alternate promoter in human liver cells." in: The Journal of clinical investigation, Vol. 118, Issue 2, pp. 695-709, (2008) (PubMed).

  • 抗原

    HNF1B (HNF1 Homeobox B (HNF1B))

    别名

    HNF1B

    背景

    TCF2, VHNF1, transcription factor 2, hepatic LF-B3 variant hepatic nuclear factor, FJHN, HNF1B, HNF1beta, HNF2, LFB3, MODY5, transcription factor 2

    基因ID

    6928, 21410, 25640

    NCBI登录号

    NP_000449, NP_001159395

    途径

    Hormone Transport, Stem Cell Maintenance, Tube Formation
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