s100a4 抗体 (C-Term)
Quick Overview for s100a4 抗体 (C-Term) (ABIN334485)
抗原
See all s100a4 (S100A4) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- S100A4 / CAPL
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序列
- CNEFFEGFPD KQP
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特异性
- Both variants (NP_002952.1, NP_062427.1) represent identical protein.
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交叉反应
- 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence CNEFFEGFPDKQP, from the C Terminus of the protein sequence according to NP_002952.1, NP_062427.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Small Intestine shows staining of lymphatic cells in the lamina propria. Recommended concentration: 1-2 μg/mL.
Western Blot: Approx 13 kDa band observed in Human Peripheral blood mononuclear cells lysates (calculated MW of 11.7 kDa according to NP_002952.1). Recommended concentration: 0.05-0.2 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:128000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Comparative secretome analysis of epithelial and mesenchymal subpopulations of head and neck squamous cell carcinoma identifies S100A4 as a potential therapeutic target." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 12, pp. 3778-92, (2013) (PubMed).
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: "Comparative secretome analysis of epithelial and mesenchymal subpopulations of head and neck squamous cell carcinoma identifies S100A4 as a potential therapeutic target." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 12, pp. 3778-92, (2013) (PubMed).
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- s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))
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别名
- S100A4
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背景
- S100A4, CAPL, S100 calcium binding protein A4, 18A2, 42A, MTS1, P9KA, PEL98, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S100 calcium-binding protein A4 (calcium pr
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基因ID
- 6275, 20198, 24615
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NCBI登录号
- NP_002952, NP_062427
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途径
- S100 Proteins
抗原
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