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PRPF31 抗体 (Internal Region)

This 山羊 多克隆 anti-PRPF31 antibody specifically detects PRPF31 in WB, ELISA, IF 和 FACS. The antibody is reactive with 人 samples.
产品编号 ABIN334465
发货至: 中国
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Quick Overview for PRPF31 抗体 (Internal Region) (ABIN334465)

抗原

See all PRPF31 抗体
PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

适用

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宿主

  • 49
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山羊

克隆类型

  • 32
  • 25
多克隆

标记

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This PRPF31 antibody is un-conjugated

应用范围

  • 49
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Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    PRPF31

    序列

    KELGNSLDKC KNNEN

    交叉反应

    Cow, 犬, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence KELGNSLDKCKNNEN, from the internal region of the protein sequence according to NP_056444.3.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 60 kDa band observed in nuclear lysates of cell lines A431, HeLa, HEK293 and NIH3T3 (calculated MW of 55.4 kDa according to Human NP_056444.3 and Mouse NP_081604.3). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at roo

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HEK293 and HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

    别名

    PRPF31

    背景

    PRPF31, PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae), DKFZp566J153, NY-BR-99, PRP31, RP11 , PRP31 pre-mRNA processing factor 31 homolog (yeast), pre-mRNA processing factor 31 homolog, pre-mRNA processing factor 31 homolog (yeast)

    基因ID

    26121, 68988

    NCBI登录号

    NP_056444

    途径

    Ribonucleoprotein Complex Subunit Organization
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