MGLL 抗体 (Internal Region)
Quick Overview for MGLL 抗体 (Internal Region) (ABIN334436)
抗原
See all MGLL 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- Monoglyceride Lipase
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序列
- QDLPHLVNAD GQY
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特异性
- This antibody is expected to recognize both reported isoforms (NP_009214.1, NP_001003794.1).
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-QDLPHLVNADGQY, from the internal region (near N Terminus) of the protein sequence according to NP_009214.1, NP_001003794.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Liver, expression of MGLL is detected in hepatocytes. Recommended concentration: 5 μg/mL.
Western Blot: Approx 35 kDa band observed in Mouse Adipose lysates and in preliminary testing of Human, Cerebellum lysate and in lysate of cell line NIH3T3 (calculated MW of 33.3 kDa according to NP_001003794.1). Recommended concentration: 0.3-1 μg/mL. Pri
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- MGLL (Monoglyceride Lipase (MGLL))
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别名
- MGLL
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背景
- Monoglyceride lipase, HU-K5, MGLL, MGL, lysophospholipase-like
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基因ID
- 11343, 23945, 29254
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NCBI登录号
- NP_009214, NP_001003794
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途径
- Regulation of G-Protein Coupled Receptor Protein Signaling, Lipid Metabolism
抗原
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