GAD 抗体 (Internal Region)
(1 reference)Quick Overview for GAD 抗体 (Internal Region) (ABIN334405)
抗原
See all GAD (GAD1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- GAD1 (isoform GAD67)
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序列
- PDSPQRREKL HK
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特异性
- This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
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交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-PDSPQRREKLHK, from the internal region of the protein sequence according to NP_000808.2.
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亚型
- IgG
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anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 1-200) antibody
GAD1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (N-Term) antibodyGAD1 适用: 人 WB, IHC, ICC, IHC (p), IF, IHC (fro), IHC (ff), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 1-97) antibodyGAD1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 455-594) antibodyGAD1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 72-135) antibodyGAD1 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal GAD1-2391 unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibodyGAD1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 72-135) antibodyGAD1 适用: 人 WB, ELISA, FACS, Coat 宿主: 小鼠 Monoclonal GAD1-2563 unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 14-122) antibodyGAD1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibodyGAD1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Approx 65-70 kDa band observed in Human Cerebral Cortex lysates (calculated MW of 66.9 kDa according to NP_000808.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme." in: Scientific reports, Vol. 8, Issue 1, pp. 15470, (2018) (PubMed).
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: "A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme." in: Scientific reports, Vol. 8, Issue 1, pp. 15470, (2018) (PubMed).
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- GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
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别名
- GAD1
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背景
- GAD1, glutamate decarboxylase 1 (brain, 67 kDa), FLJ45882, GAD, SCP, OTTHUMP00000041055, glutamate decarboxylase 1, glutamate decarboxylase 1 (brain, 67kD), GAD67, GAD25
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基因ID
- 2571, 14415, 24379
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NCBI登录号
- NP_000808
抗原
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