Fascin 2 抗体 (Internal Region)
Quick Overview for Fascin 2 抗体 (Internal Region) (ABIN334397)
抗原
See all Fascin 2 (FSCN2) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- Fascin 2
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序列
- CHHRGSNQLD TNR
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特异性
- This antibody is expected to recognize both reported isoforms (NP_001070650.1 and NP_036550.1).
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交叉反应
- 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence CHHRGSNQLDTNR, from the internal region of the protein sequence according to NP_001070650.1, NP_036550.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Mouse Brain. Recommended concentration: 4 μg/mL. This product was successfully used on sections of mouse cochlea as descibed in Shin et al, J Neurosci. 2010 Jul 21,30(29):9683-94, PMID: 20660251.
Peptide ELISA: antibody detection limit dilution 1:64000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Single-cell RNA-Seq resolves cellular complexity in sensory organs from the neonatal inner ear." in: Nature communications, Vol. 6, pp. 8557, (2015) (PubMed).
: "HCN1 and HCN2 proteins are expressed in cochlear hair cells: HCN1 can form a ternary complex with protocadherin 15 CD3 and F-actin-binding filamin A or can interact with HCN2." in: The Journal of biological chemistry, Vol. 287, Issue 45, pp. 37628-46, (2012) (PubMed).
: "The R109H variant of fascin-2, a developmentally regulated actin crosslinker in hair-cell stereocilia, underlies early-onset hearing loss of DBA/2J mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 29, pp. 9683-94, (2010) (PubMed).
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: "Single-cell RNA-Seq resolves cellular complexity in sensory organs from the neonatal inner ear." in: Nature communications, Vol. 6, pp. 8557, (2015) (PubMed).
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- Fascin 2 (FSCN2)
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别名
- FSCN2
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背景
- Fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus), RFSN, RP30, fascin 2, retinal fascin,
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基因ID
- 25794, 238021, 303741
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NCBI登录号
- NP_001070650, NP_036550
抗原
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