alpha 2 Antiplasmin 抗体 (Internal Region)
Quick Overview for alpha 2 Antiplasmin 抗体 (Internal Region) (ABIN334343)
抗原
See all alpha 2 Antiplasmin (SERPINF2) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- Alpha2-antiplasmin
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序列
- KDFLQSLKGF PRGDK
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特异性
- This antibody is expected to recognise both reported isoforms (NP_000925.2, NP_001159393.1). Reported variants represent identical protein (NP_000925.2, NP_001159392.1).
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-KDFLQSLKGFPRGDK, from the internal region of the protein sequence according to NP_000925.2, NP_001159393.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Liver shows spotty cytoplasm staining in hepatocytes. Recommended concentration: 3-5 μg/mL.
Western Blot: Approx 70 kDa band observed in lysates of cell line HepG2 (calculated MW of 54.6 kDa according to NP_000925.2). The observed molecular weight is observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at r
Peptide ELISA: antibody detection limit dilution 1:16000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "On the localization of the cleavage site in human alpha-2-antiplasmin, involved in the generation of the non-plasminogen binding form." in: Journal of thrombosis and haemostasis : JTH, Vol. 18, Issue 5, pp. 1162-1170, (2020) (PubMed).
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: "On the localization of the cleavage site in human alpha-2-antiplasmin, involved in the generation of the non-plasminogen binding form." in: Journal of thrombosis and haemostasis : JTH, Vol. 18, Issue 5, pp. 1162-1170, (2020) (PubMed).
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- alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))
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别名
- SERPINF2
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背景
- SERPINF2, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2, A2AP, AAP, ALPHA-2-PI, API, PLI, alpha-2-plasmin inhibitor, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment
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基因ID
- 5345
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NCBI登录号
- NP_000925, NP_001159393
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途径
- Regulation of Systemic Arterial Blood Pressure by Hormones
抗原
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