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alpha 2 Antiplasmin 抗体 (Internal Region)

This anti-alpha 2 Antiplasmin antibody is a 山羊 多克隆 antibody detecting alpha 2 Antiplasmin in WB, IHC, ELISA, IF 和 FACS. Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN334343
发货至: 中国

Quick Overview for alpha 2 Antiplasmin 抗体 (Internal Region) (ABIN334343)

抗原

See all alpha 2 Antiplasmin (SERPINF2) 抗体
alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This alpha 2 Antiplasmin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    Alpha2-antiplasmin

    序列

    KDFLQSLKGF PRGDK

    特异性

    This antibody is expected to recognise both reported isoforms (NP_000925.2, NP_001159393.1). Reported variants represent identical protein (NP_000925.2, NP_001159392.1).

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-KDFLQSLKGFPRGDK, from the internal region of the protein sequence according to NP_000925.2, NP_001159393.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Liver shows spotty cytoplasm staining in hepatocytes. Recommended concentration: 3-5 μg/mL.

    Western Blot: Approx 70 kDa band observed in lysates of cell line HepG2 (calculated MW of 54.6 kDa according to NP_000925.2). The observed molecular weight is observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at r

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Abdul, Dekkers, Ariëns, Leebeek, Rijken, Uitte de Willige: "On the localization of the cleavage site in human alpha-2-antiplasmin, involved in the generation of the non-plasminogen binding form." in: Journal of thrombosis and haemostasis : JTH, Vol. 18, Issue 5, pp. 1162-1170, (2020) (PubMed).

  • 抗原

    alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

    别名

    SERPINF2

    背景

    SERPINF2, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2, A2AP, AAP, ALPHA-2-PI, API, PLI, alpha-2-plasmin inhibitor, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment

    基因ID

    5345

    NCBI登录号

    NP_000925, NP_001159393

    途径

    Regulation of Systemic Arterial Blood Pressure by Hormones
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