DPP7 抗体
Quick Overview for DPP7 抗体 (ABIN332833)
抗原
See all DPP7 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Recognizes human DPPII. Species sequence homology: chimpanzee 99 % , mouse 81 %, rat 80 %, dog 79 % and chicken 65 %.
-
纯化方法
- Protein G purified
-
免疫原
-
NS0-derived recombinant human rhDPPII.
Type of Immunogen: Recombinant protein -
亚型
- IgG2a
-
-
-
-
应用备注
-
Approved: ELISA, Neut, WB (1 - 2 μg/mL)
Usage: Suitable for use in ELISA, Neutralization and Western blot. Neutralization-preincubated with the. enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then. assayed with a fluorogenic peptide substrate. 50 % of the proteolytic activity was inhibited by. the antibody at approximately 0.12 μg/mL (IC50) under conditions in which the enzyme was. present at 0.1 μg/mL and the substrate concentration was 10uM. Considering the molecular. masses of the enzyme (53kD) and the antibody (150kD), IC50 was achieved at. approximately 1:2 Molar ratio of the antibody to the enzyme. Western blot: 1-2 μg/mL The detection limit is ~10 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Chemiluminescent detection with. WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.
-
浓度
- Lot specific
-
缓冲液
- Lyophilized from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.
-
-
- DPP7 (Dipeptidyl-Peptidase 7 (DPP7))
-
别名
- DPP2 / DPP7
-
背景
-
Name/Gene ID: DPP7
Subfamily: Serine S28
Family: Protease
Synonyms: DPP7, Carboxytripeptidase, Dipeptidyl aminopeptidase II, Dipeptidyl peptidase 2, DPPII, Dipeptidyl arylamidase II, Dipeptidyl-peptidase 7, Dipeptidyl-peptidase II, Dipeptidylpeptidase 7, Dipeptidyl peptidase 7, Dipeptidyl peptidase II, DPP II, DPP2, QPP -
基因ID
- 29952
-
UniProt
- Q9UHL4
抗原
-