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DPP7 抗体

This 大鼠 单克隆 antibody specifically detects DPP7 in WB, ELISA 和 Neut. It exhibits reactivity toward 人.
产品编号 ABIN332833
发货至: 中国
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北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for DPP7 抗体 (ABIN332833)

抗原

See all DPP7 抗体
DPP7 (Dipeptidyl-Peptidase 7 (DPP7))

适用

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宿主

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大鼠

克隆类型

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单克隆

标记

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This DPP7 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 特异性

    Recognizes human DPPII. Species sequence homology: chimpanzee 99 % , mouse 81 %, rat 80 %, dog 79 % and chicken 65 %.

    纯化方法

    Protein G purified

    免疫原

    NS0-derived recombinant human rhDPPII.

    Type of Immunogen: Recombinant protein

    亚型

    IgG2a
  • 应用备注

    Approved: ELISA, Neut, WB (1 - 2 μg/mL)

    Usage: Suitable for use in ELISA, Neutralization and Western blot. Neutralization-preincubated with the. enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then. assayed with a fluorogenic peptide substrate. 50 % of the proteolytic activity was inhibited by. the antibody at approximately 0.12 μg/mL (IC50) under conditions in which the enzyme was. present at 0.1 μg/mL and the substrate concentration was 10uM. Considering the molecular. masses of the enzyme (53kD) and the antibody (150kD), IC50 was achieved at. approximately 1:2 Molar ratio of the antibody to the enzyme. Western blot: 1-2 μg/mL The detection limit is ~10 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Chemiluminescent detection with. WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.
  • 抗原

    DPP7 (Dipeptidyl-Peptidase 7 (DPP7))

    别名

    DPP2 / DPP7

    背景

    Name/Gene ID: DPP7
    Subfamily: Serine S28
    Family: Protease

    Synonyms: DPP7, Carboxytripeptidase, Dipeptidyl aminopeptidase II, Dipeptidyl peptidase 2, DPPII, Dipeptidyl arylamidase II, Dipeptidyl-peptidase 7, Dipeptidyl-peptidase II, Dipeptidylpeptidase 7, Dipeptidyl peptidase 7, Dipeptidyl peptidase II, DPP II, DPP2, QPP

    基因ID

    29952

    UniProt

    Q9UHL4
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