Actin 抗体
Quick Overview for Actin 抗体 (ABIN331865)
抗原
See all Actin 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Reacts with all six known vertebrate isoforms of actin (MW ~42kD). It also reacts with two cytoplasmic actins (beta, gamma) which are highly homologous to one another, but which differ from the muscle actins by about 25 amino acid residues. This antibody is highly recommended for monitoring total protein load on Western blots. Species cross-reactivity: Human, bovine, mouse, canine, porcine, rabbit, rat, chicken, Dictyostelium discoideum, Physarum polycephalum.
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纯化方法
- Protein G purified
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免疫原
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Chicken gizzard actin.
Type of Immunogen: Purified protein -
亚型
- IgG1 kappa
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anti-Actin antibody
适用: 植物 WB 宿主: 小鼠 Monoclonal Q30 unconjugated
anti-Actin (AA 165-260) antibody适用: 人, 大鼠, 小鼠, 兔, 犬 WB, ELISA, IHC (p), IF (cc), IF (p), FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Actin antibody适用: 人, 大鼠, 小鼠, 小鸡, 犬, Cow, Pig, 豚鼠 WB, ELISA, IHC (p), IF (cc), IF (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Actin (C-Term) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Actin antibody适用: Oryza sativa, Barley, Arabidopsis, Nicotiana tabacum WB 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 367-377) antibody适用: 人, 大鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 21-70) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 38-67), (N-Term) antibody适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 189-218) antibody适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: IHC, IHC-P (0.5 μg/mL), IP, WB (0.5 μg/mL)
Usage: Suitable for use in Immunoprecipitation, Immunohistochemistry and Western Blot. Immunoprecipitation (Denatured verified): 2 μg/mg protein lysate. Use Protein G. Western Blot: 0.5 μg/mL for 2hrs at room temperature. Immunohistochemistry (formalin/paraffin): 1:400 for 30 min. Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Positive control: HeLa cells, skeletal muscle. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Chicken
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM PBS, pH 7.4.
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储存液
- Azide free
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- Actin
抗原
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