Actin 抗体 (N-Term)
Quick Overview for Actin 抗体 (N-Term) (ABIN331838)
抗原
See all Actin 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 50-70, N-Term
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特异性
- Recognizes all six isoforms of vertebrate actin, globular (G) and filamentous (F) forms. This clone is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. Labels myotubes and stains myoblasts and fibroblasts. Does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. Species cross-reactivity: All species.
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纯化方法
- Protein A purified
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免疫原
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Purified chicken gizzard actin. Epitope: Appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70.
Type of Immunogen: Purified protein -
亚型
- IgG2b kappa
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anti-Actin antibody
适用: 植物 WB 宿主: 小鼠 Monoclonal Q30 unconjugated
anti-Actin (AA 165-260) antibody适用: 人, 大鼠, 小鼠, 兔, 犬 WB, ELISA, IHC (p), IF (cc), IF (p), FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Actin antibody适用: 人, 大鼠, 小鼠, 小鸡, 犬, Cow, Pig, 豚鼠 WB, ELISA, IHC (p), IF (cc), IF (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Actin (C-Term) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Actin antibody适用: Oryza sativa, Barley, Arabidopsis, Nicotiana tabacum WB 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 367-377) antibody适用: 人, 大鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 21-70) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 38-67), (N-Term) antibody适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 189-218) antibody适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, ICC (1 - 20 μg/mL), IHC, IHC-P, WB
Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA. Immunohistochemistry: 10 μg/mL (paraffin embedded, 4 % formaldehyde, 3 % glutaraldehyde, sodium cacodylate treated sections). Visualized using a donkey anti-mouse IgG (Cy3). Positive staining pattern reflects both membrane and cytoplasmic staining. ELISA: Shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoideum and Physarum polycephalum in ELISA. -
说明
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Target Species of Antibody: Chicken
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Actin
抗原
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