FOXH1 抗体 (AA 164-272)
Quick Overview for FOXH1 抗体 (AA 164-272) (ABIN329971)
抗原
See all FOXH1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 164-272
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特异性
- Recognizes human FoxH1. Species sequence homology: mouse 55 % . less than 1 % cross-reactivity with rhFoxB2.
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纯化方法
- Affinity purified
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免疫原
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E. coli-derived recombinant human rhFoxH1 (aa 164 - 272).
Type of Immunogen: Recombinant protein -
亚型
- IgG
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anti-Forkhead Box H1 (FOXH1) (N-Term) antibody
FOXH1 适用: 人 WB, IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box H1 (FOXH1) (AA 313-334) antibodyFOXH1 适用: 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box H1 (FOXH1) (AA 153-269) antibodyFOXH1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box H1 (FOXH1) antibodyFOXH1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box H1 (FOXH1) (AA 290-322), (C-Term) antibodyFOXH1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB51002 unconjugated
anti-Forkhead Box H1 (FOXH1) (AA 1-365) antibodyFOXH1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box H1 (FOXH1) (Internal Region) antibodyFOXH1 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box H1 (FOXH1) (N-Term) antibodyFOXH1 适用: 人, Cow, Pig, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box H1 (FOXH1) (AA 241-290) antibodyFOXH1 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Western blot, Immunocytochemistry and Direct ELISA. Western blot: 0.1-0.2 μg/mL. The detection limit is ~5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect human FoxH1 in human BG0IV stem cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at room temperature for 1 hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.2 ng/well. Hybridoma: Produced in sheep immunized with purified, E. coli-derived, recombinant human FoxH1. (rhFoxH1, aa 164-272, Accession # O75593). -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute, 500 μL sterile PBS to a concentration of 0.2 mg/mL.
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浓度
- Lot specific
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缓冲液
- Lyophilized powder from PBS, 5 % trehalose. Reconstitute, 500 μLsterile PBS to a concentration of 0.2 mg/mL.
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- FOXH1 (Forkhead Box H1 (FOXH1))
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别名
- FOXH1
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背景
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Name/Gene ID: FOXH1
Family: Transcription factor
Synonyms: FOXH1, Fast-2, FAST2, FAST-1, FAST1, Forkhead box H1, Forkhead box protein H1, HFAST-1 -
基因ID
- 8928
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UniProt
- O75593
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
抗原
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