MLX 抗体 (AA 197-298)
Quick Overview for MLX 抗体 (AA 197-298) (ABIN328581)
抗原
See all MLX 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 197-298
-
特异性
- Recognizes human MLX. Species sequence homology: 99 % identical to mouse and canine MLX.
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
E. coli-derived recombinant human rhMLX (aa 197-298).
Type of Immunogen: Recombinant protein -
亚型
- IgG
-
-
-
-
应用备注
-
Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Western blot, Immunocytochemistry and ELISA. Western blot: 0.1-0.2 μg/mL. The detection limit is ~2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL will detect human MLX in serum stimulated W138 human fetal lung fibroblasts. Fix cells with PBS containing 4 % paraformaldehyde for 20 minutes at RT and block with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, incubate cells with diluted primary antibody for 3 hours at RT. followed by NorthernLights 557 donkey anti-goat lgG at RT for 1 hour. Between each step, wash cells with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.3 ng/well. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitute, 0.5 mL PBS to a concentration of 0.2 mg/mL.
-
浓度
- Lot specific
-
缓冲液
- Lyophilized powder from PBS, 5 % trehalose. Reconstitute, 0.5 mL PBS to a concentration of 0.2 mg/mL.
-
-
- MLX (MAX-Like Protein X (MLX))
-
别名
- MLX / TCFL4
-
背景
-
Name/Gene ID: MLX
Synonyms: MLX, BHLHd13, BigMax protein, MXD7, MAD7, Max-like bHLHZip protein, TCFL4, MLX, MAX dimerization protein, Protein BigMax, Max-like protein X, Transcription factor-like 4 -
基因ID
- 6945
-
UniProt
- Q9UH92
抗原
-