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MLX 抗体 (AA 197-298)

The 山羊 多克隆 anti-MLX antibody (ABIN328581) specifically detects MLX in WB, ELISA 和 ICC. The antibody is reactive with 人 samples.
产品编号 ABIN328581
发货至: 中国
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Quick Overview for MLX 抗体 (AA 197-298) (ABIN328581)

抗原

See all MLX 抗体
MLX (MAX-Like Protein X (MLX))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This MLX antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
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    AA 197-298

    特异性

    Recognizes human MLX. Species sequence homology: 99 % identical to mouse and canine MLX.

    纯化方法

    Immunoaffinity purified

    免疫原

    E. coli-derived recombinant human rhMLX (aa 197-298).

    Type of Immunogen: Recombinant protein

    亚型

    IgG
  • 应用备注

    Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

    Usage: Suitable for use in Western blot, Immunocytochemistry and ELISA. Western blot: 0.1-0.2 μg/mL. The detection limit is ~2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL will detect human MLX in serum stimulated W138 human fetal lung fibroblasts. Fix cells with PBS containing 4 % paraformaldehyde for 20 minutes at RT and block with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, incubate cells with diluted primary antibody for 3 hours at RT. followed by NorthernLights 557 donkey anti-goat lgG at RT for 1 hour. Between each step, wash cells with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.3 ng/well.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute, 0.5 mL PBS to a concentration of 0.2 mg/mL.

    浓度

    Lot specific

    缓冲液

    Lyophilized powder from PBS, 5 % trehalose. Reconstitute, 0.5 mL PBS to a concentration of 0.2 mg/mL.
  • 抗原

    MLX (MAX-Like Protein X (MLX))

    别名

    MLX / TCFL4

    背景

    Name/Gene ID: MLX

    Synonyms: MLX, BHLHd13, BigMax protein, MXD7, MAD7, Max-like bHLHZip protein, TCFL4, MLX, MAX dimerization protein, Protein BigMax, Max-like protein X, Transcription factor-like 4

    基因ID

    6945

    UniProt

    Q9UH92
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