IL17F 抗体 (AA 31-163)
Quick Overview for IL17F 抗体 (AA 31-163) (ABIN327416)
抗原
See all IL17F 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 31-163
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特异性
- Recognizes human IL-17F. In direct ELISAs and Western Blots this antibody does not cross-react with rhIL-17A, B, C, D, or E.
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纯化方法
- Protein G purified
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免疫原
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E. coli-derived rhIL-17F corresponding to aa 31-163 according to Q96PD4.
Type of Immunogen: Recombinant protein -
亚型
- IgG2b
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应用备注
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Approved: ELISA, Flo
Usage: Suitable for use in ELISA and Flow Cytometry. The detection limit is ~25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry (Intracellular): 25 μg/mL. Add 10 μL to 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. For intracellular staining to detect IL-17F, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. This antibody was tested using PMA and Ca2+ ionomycin-treated PBMCs. ELISA (Direct): 0.5-1.0 μg/mL. The detection limit is ~1 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 200 μL sterile PBS
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose.
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
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- IL17F (Interleukin 17F (IL17F))
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别名
- IL17F
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背景
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Name/Gene ID: IL17F
Synonyms: IL17F, CANDF6, Cytokine ML-1, IL-17F, Interleukin-17F, Interleukin 17F, Interleukin-24, ML-1, ML1, Mutant IL-17F -
基因ID
- 112744
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UniProt
- Q96PD4
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途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity
抗原
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