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IL17F 抗体 (AA 31-163)

The 小鼠 单克隆 anti-IL17F antibody is suitable to detect IL17F in samples from 人. It has been validated for ELISA 和 FACS.
产品编号 ABIN327416
发货至: 中国
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北京 101111
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Room 801-803
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Quick Overview for IL17F 抗体 (AA 31-163) (ABIN327416)

抗原

See all IL17F 抗体
IL17F (Interleukin 17F (IL17F))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This IL17F antibody is un-conjugated

应用范围

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ELISA, Flow Cytometry (FACS)

克隆位点

197301
  • 抗原表位

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    AA 31-163

    特异性

    Recognizes human IL-17F. In direct ELISAs and Western Blots this antibody does not cross-react with rhIL-17A, B, C, D, or E.

    纯化方法

    Protein G purified

    免疫原

    E. coli-derived rhIL-17F corresponding to aa 31-163 according to Q96PD4.

    Type of Immunogen: Recombinant protein

    亚型

    IgG2b
  • 应用备注

    Approved: ELISA, Flo

    Usage: Suitable for use in ELISA and Flow Cytometry. The detection limit is ~25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry (Intracellular): 25 μg/mL. Add 10 μL to 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. For intracellular staining to detect IL-17F, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. This antibody was tested using PMA and Ca2+ ionomycin-treated PBMCs. ELISA (Direct): 0.5-1.0 μg/mL. The detection limit is ~1 ng/well.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    200 μL sterile PBS

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS, 5 % trehalose.

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    -20 °C

    储存方法

    Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
  • 抗原

    IL17F (Interleukin 17F (IL17F))

    别名

    IL17F

    背景

    Name/Gene ID: IL17F

    Synonyms: IL17F, CANDF6, Cytokine ML-1, IL-17F, Interleukin-17F, Interleukin 17F, Interleukin-24, ML-1, ML1, Mutant IL-17F

    基因ID

    112744

    UniProt

    Q96PD4

    途径

    Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity
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