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IL15RA 抗体 (Extracellular Domain)

IL15RA 适用: 小鼠 WB, ELISA, FACS, Neut, Func 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN327397
发货至: 中国
  • 抗原 See all IL15RA 抗体
    IL15RA (Interleukin 15 Receptor, alpha (IL15RA))
    抗原表位
    • 15
    • 7
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain
    适用
    • 43
    • 28
    • 14
    • 6
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    小鼠
    宿主
    • 39
    • 4
    • 2
    • 1
    山羊
    克隆类型
    • 39
    • 7
    多克隆
    标记
    • 21
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL15RA antibody is un-conjugated
    应用范围
    • 43
    • 21
    • 13
    • 13
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Neutralization (Neut), Functional Studies (Func)
    特异性
    Recognizes mouse IL-15Ra.
    纯化方法
    Immunoaffinity purified
    免疫原
    NS0-derived rmIL-15Ra extracellular domain.

    Type of Immunogen: Recombinant protein
    亚型
    IgG
    Top Product
    Discover our top product IL15RA Primary Antibody
  • 应用备注
    Approved: ELISA, Flo, Func, Neut, WB

    Usage: Neutralization of bioactivity, Direct ELISA, Western Blot, Flow Cytometry. Neutralization of Mouse IL-15 R Bioactivity: The exact concentration of antibody required to neutralize the mouse cell surface IL-15 R mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-15 R mediated IL-15 response on a responsive cell line, at a specific IL-15 concentration. As shown in Figures 1 and 2 on the next page, the ND50 for this lot of anti-mouse IL-15 R antibody was determined to be approximately 0.3-1.5 μg/mL in the presence of 0.8 ng/mL of human IL-15, using the CTLL-2 cell proliferation assay. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 0.1 ng/well. In this format, this antibody shows approximately 5 % cross-reactivity with rhIL-15 R and less than 1 % cross-reactivity with rhIL-2 R, rmIL-2 R and rmIL-2 R. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x106 with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry.
    说明

    Target Species of Antibody: Mouse

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.
  • 抗原
    IL15RA (Interleukin 15 Receptor, alpha (IL15RA))
    别名
    IL15RA (IL15RA 产品)
    别名
    IL15RA antibody, CD215 antibody, AA690181 antibody, IL-15RA antibody, interleukin 15 receptor subunit alpha antibody, interleukin 15 receptor, alpha chain antibody, IL15RA antibody, Il15ra antibody
    背景
    Name/Gene ID: IL15RA
    Family: Interleukin

    Synonyms: IL15RA, CD215 antigen, IL-15RA, IL-15 receptor subunit alpha, IL-15R-alpha, Interleukin 15 receptor, alpha, CD215
    基因ID
    3601
    UniProt
    Q13261
    途径
    JAK/STAT Signaling
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