IL15RA 抗体 (Extracellular Domain)
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- 抗原 See all IL15RA 抗体
- IL15RA (Interleukin 15 Receptor, alpha (IL15RA))
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抗原表位
- Extracellular Domain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This IL15RA antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Neutralization (Neut), Functional Studies (Func)
- 特异性
- Recognizes mouse IL-15Ra.
- 纯化方法
- Immunoaffinity purified
- 免疫原
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NS0-derived rmIL-15Ra extracellular domain.
Type of Immunogen: Recombinant protein - 亚型
- IgG
- Top Product
- Discover our top product IL15RA Primary Antibody
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- 应用备注
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Approved: ELISA, Flo, Func, Neut, WB
Usage: Neutralization of bioactivity, Direct ELISA, Western Blot, Flow Cytometry. Neutralization of Mouse IL-15 R Bioactivity: The exact concentration of antibody required to neutralize the mouse cell surface IL-15 R mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-15 R mediated IL-15 response on a responsive cell line, at a specific IL-15 concentration. As shown in Figures 1 and 2 on the next page, the ND50 for this lot of anti-mouse IL-15 R antibody was determined to be approximately 0.3-1.5 μg/mL in the presence of 0.8 ng/mL of human IL-15, using the CTLL-2 cell proliferation assay. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 0.1 ng/well. In this format, this antibody shows approximately 5 % cross-reactivity with rhIL-15 R and less than 1 % cross-reactivity with rhIL-2 R, rmIL-2 R and rmIL-2 R. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x106 with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry. - 说明
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Target Species of Antibody: Mouse
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.
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- 抗原
- IL15RA (Interleukin 15 Receptor, alpha (IL15RA))
- 别名
- IL15RA (IL15RA 产品)
- 背景
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Name/Gene ID: IL15RA
Family: Interleukin
Synonyms: IL15RA, CD215 antigen, IL-15RA, IL-15 receptor subunit alpha, IL-15R-alpha, Interleukin 15 receptor, alpha, CD215 - 基因ID
- 3601
- UniProt
- Q13261
- 途径
- JAK/STAT Signaling
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