IL15RA 抗体 (Extracellular Domain)
Quick Overview for IL15RA 抗体 (Extracellular Domain) (ABIN327397)
抗原
See all IL15RA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Extracellular Domain
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特异性
- Recognizes mouse IL-15Ra.
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纯化方法
- Immunoaffinity purified
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免疫原
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NS0-derived rmIL-15Ra extracellular domain.
Type of Immunogen: Recombinant protein -
亚型
- IgG
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anti-Interleukin 15 Receptor, alpha (IL15RA) (AA 31-205) antibody
IL15RA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5F10D9 unconjugated
anti-Interleukin 15 Receptor, alpha (IL15RA) (AA 31-205) antibodyIL15RA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 15 Receptor, alpha (IL15RA) antibodyIL15RA 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 15 Receptor, alpha (IL15RA) (AA 31-130) antibodyIL15RA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C5 unconjugated
anti-Interleukin 15 Receptor, alpha (IL15RA) (AA 31-174) antibodyIL15RA 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, FACS, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 15 Receptor, alpha (IL15RA) (AA 31-130) antibodyIL15RA 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin 15 Receptor, alpha (IL15RA) (Internal Region) antibodyIL15RA 适用: 人, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 15 Receptor, alpha (IL15RA) antibodyIL15RA 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal unconjugated
anti-Interleukin 15 Receptor, alpha (IL15RA) (Extracellular Domain) antibody (Carboxyfluorescein (CFS))IL15RA 适用: 小鼠 FACS 宿主: 山羊 Polyclonal Carboxyfluorescein (CFS)
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应用备注
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Approved: ELISA, Flo, Func, Neut, WB
Usage: Neutralization of bioactivity, Direct ELISA, Western Blot, Flow Cytometry. Neutralization of Mouse IL-15 R Bioactivity: The exact concentration of antibody required to neutralize the mouse cell surface IL-15 R mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-15 R mediated IL-15 response on a responsive cell line, at a specific IL-15 concentration. As shown in Figures 1 and 2 on the next page, the ND50 for this lot of anti-mouse IL-15 R antibody was determined to be approximately 0.3-1.5 μg/mL in the presence of 0.8 ng/mL of human IL-15, using the CTLL-2 cell proliferation assay. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 0.1 ng/well. In this format, this antibody shows approximately 5 % cross-reactivity with rhIL-15 R and less than 1 % cross-reactivity with rhIL-2 R, rmIL-2 R and rmIL-2 R. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x106 with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.
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- IL15RA (Interleukin 15 Receptor, alpha (IL15RA))
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别名
- IL15RA
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背景
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Name/Gene ID: IL15RA
Family: Interleukin
Synonyms: IL15RA, CD215 antigen, IL-15RA, IL-15 receptor subunit alpha, IL-15R-alpha, Interleukin 15 receptor, alpha, CD215 -
基因ID
- 3601
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UniProt
- Q13261
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途径
- JAK/STAT Signaling
抗原
-
