IGFBPL1 抗体 (AA 18-270)
-
- 抗原 See all IGFBPL1 products
- IGFBPL1 (Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBPL1))
-
抗原表位
- AA 18-270
-
适用
- 小鼠
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This IGFBPL1 antibody is un-conjugated
-
应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- Recognizes mouse IGFBP-L1. (aa 18-270) Cross-reactivity: rhIGFBP-L1:10 % and rmIGFBP-7 less than 1 %. Species sequence homology: Rat 96 %, human 79 %.
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
NS0-derived recombinant rat rmIGFBP-L1.
Type of Immunogen: Recombinant protein - 亚型
- IgG
-
-
- 应用备注
-
Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Western blot, Immunohistochemistry, and Direct ELISA. Western Blot: 0.1-0.2 μg/mL. The detection limit is ~2 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Immunohistochemistry: 10 μg/mL detects IGFBP-L1 in cryosections of E13.5 mouse brain. Tissue was fixed overnight in 4 % PFA+14 % picric acid in PBS at 4? C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 minutes at RT. After. blocking, sections were incubated with primary antibody for 3 hours at RT followed by NorthernLights 557 donkey anti-goat lgG at RT for 1 hour. Between each step, sections were. washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.5 ng/well. - 说明
-
Target Species of Antibody: Mouse
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitute, 0.5 mL sterile PBS.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, 5 % trehalose. Reconstitute, 0.5 mL sterile PBS.
-
- 抗原
- IGFBPL1 (Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBPL1))
- 别名
- IGFBPL1 (IGFBPL1 产品)
- 别名
- IGFBP-RP4 antibody, IGFBPRP4 antibody, bA113O24.1 antibody, 2810011G06Rik antibody, 2810453O06Rik antibody, IGFBPL antibody, insulin like growth factor binding protein like 1 antibody, insulin-like growth factor binding protein-like 1 antibody, IGFBPL1 antibody, Igfbpl1 antibody
- 背景
-
Name/Gene ID: IGFBPL1
Synonyms: IGFBPL1, BA113O24.1, IGFBP-RP4, IGFBP-related protein 10, IGFBPRP4 - 基因ID
- 347252
- UniProt
- Q8WX77
-